1FH5
CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE MONOCLONAL ANTIBODY MAK33
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-08 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 58.910, 65.660, 73.460 |
Unit cell angles | 109.14, 106.94, 95.91 |
Refinement procedure
Resolution | 8.000 - 2.900 |
Rwork | 0.198 |
R-free | 0.24300 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.061 | 0.279 |
Number of reflections | 20143 | |
<I/σ(I)> | 10 | |
Completeness [%] | 93.7 | 87.1 * |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 4 * | na citrate, 16% PEG 8k, 16% glycerol 1.0 M NaCl, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
2 | 1 | reservoir | 1.0 (M) | ||
3 | 1 | reservoir | citrate | 50 (mM) | |
4 | 1 | reservoir | glycerol | 16 (%) |