1FGZ

GRP1 PH DOMAIN (UNLIGANDED)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	40.700, 107.600, 37.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.050
Rwork	0.223
R-free	0.27700
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.071 *	0.353
Number of reflections	10796
<I/σ(I)>	15.6
Completeness [%]	98.9	98
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	12 (%)
3	1	reservoir	sodium acetate	50 (mM)
4	1	drop		100 (mM)
5	1	reservoir	glycerol	10 (%)