1FGU
SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-01-27 |
Detector | SBC-2 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.500, 84.860, 119.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.28100 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 2.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.580 |
High resolution limit [Å] | 2.500 | 2.490 |
Rmerge | 0.052 | 0.190 |
Total number of observations | 247836 * | |
Number of reflections | 22963 | |
<I/σ(I)> | 37.1 | 4.6 |
Completeness [%] | 98.6 | 96 |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 298 | 0.1 M K,NaH2PO4 0.1 M Tris 3.8 M NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8-10 (mg/ml) | |
2 | 1 | drop | HEPES | 15 (mM) | |
3 | 1 | drop | 350 (mM) | ||
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | sodium potassium phospahte | 0.1 (M) | |
6 | 1 | reservoir | Tris | 0.1 (M) | |
7 | 1 | reservoir | 3.8 (M) |