1FGS
FOLYLPOLYGLUTAMATE SYNTHETASE FROM LACTOBACILLUS CASEI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 285 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-20 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.000, 46.100, 84.900 |
| Unit cell angles | 90.00, 107.30, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.196 * |
| R-free | 0.26500 * |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | Agrovata |
| Phasing software | MLPHARE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | 0.272 |
| Total number of observations | 39117 * | |
| Number of reflections | 15725 | |
| <I/σ(I)> | 9.5 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.3 | pH 5.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | acetate | 40 (mM) | |
| 2 | 1 | reservoir | MgATP | 5 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 4 (%) | |
| 4 | 1 | drop | FPGS | 5 (mg/ml) | |
| 5 | 1 | drop | MgATP | 10 (mM) |
