1FGQ
LIPOXYGENASE-1 (SOYBEAN) AT 100K, Q495E MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-01 |
| Detector | SBC-2 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.243, 92.551, 49.177 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.183 * |
| Rwork | 0.183 |
| R-free | 0.21580 |
| Structure solution method | IR |
| Starting model (for MR) | 1f8n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.863 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.730 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.055 | 0.398 |
| Total number of observations | 274097 * | |
| Number of reflections | 70251 | |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 98.7 | 91.1 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 * | 21 * | Minor, W., (1996) Biochemistry, 35, 10687. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | acetate | 0.1 (M) | |
| 2 | 1 | drop | PEG3400 | 4 (%(w/v)) | |
| 3 | 1 | drop | protein | 4 (mg/ml) | |
| 4 | 1 | reservoir | PEG3350 | 9 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.2 (M) |
