1FGO

LIPOXYGENASE-1 (SOYBEAN) AT 100K, Q495A MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-01
Detector	SBC-2
Spacegroup name	P 1 21 1
Unit cell lengths	94.210, 92.780, 49.450
Unit cell angles	90.00, 90.46, 90.00

Refinement procedure

Resolution	20.000 - 1.620
R-factor	0.189 *
Rwork	0.189
R-free	0.22410
Structure solution method	IR
Starting model (for MR)	1f8n
RMSD bond length	0.010
RMSD bond angle	22.923 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.680
High resolution limit [Å]	1.620	1.620
Rmerge	0.045	0.320
Total number of observations	592213 *
Number of reflections	104247
<I/σ(I)>	35.9
Completeness [%]	96.7	91.2
Redundancy	5.67	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6 *	21 *	Minor, W., (1996) Biochemistry, 35, 10687. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	acetate	0.1 (M)
2	1	drop	PEG3400	4 (%(w/v))
3	1	drop	protein	4 (mg/ml)
4	1	reservoir	PEG3350	9 (%)
5	1	reservoir	sodium acetate	0.2 (M)