1FGB

TOXIN

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1989-08-15
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	39.100, 94.500, 67.600
Unit cell angles	90.00, 96.10, 90.00

Refinement procedure

Resolution	? - 2.400
R-factor	0.171 *
Rwork	0.171
RMSD bond length	0.019
RMSD bond angle	0.037
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.380 *
Rmerge	0.029 *
Total number of observations	27472 *
Number of reflections	16703 *
Completeness [%]	85.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.75 *		Maulik, P.R., (1988) J. Biol. Chem., 263, 9499. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	CTB	3 (mg/ml)
2	1	drop	Tris-HCl	0.04 (M)
3	1	drop	beta-OG	0.375 (%)
4	1	drop	PEG3350	12 (%)
5	1	drop		0.02 (M)
6	1	drop	EDTA	0.0005 (M)
7	1	drop		0.05 (%)
8	1	reservoir	PEG3350	24 (%)