1FG9

3:1 COMPLEX OF INTERFERON-GAMMA RECEPTOR WITH INTERFERON-GAMMA DIMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Detector	PRINCETON 2K
Spacegroup name	C 1 2 1
Unit cell lengths	199.300, 114.650, 74.340
Unit cell angles	90.00, 116.27, 90.00

Refinement procedure

Resolution	6.000 - 2.900
R-factor	0.237 *
Rwork	0.237
R-free	0.30200
RMSD bond length	0.014
RMSD bond angle	2.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	2.900
Rmerge	0.056
Total number of observations	176010 *
Number of reflections	33105
Completeness [%]	80.1
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	12.5% PEG 8000, TRIS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	12.5% PEG 8000, TRIS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	12.5 (%)
2	1	reservoir	Tris-HCl