1FG5
CRYSTAL STRUCTURE OF BOVINE ALPHA-1,3-GALACTOSYLTRANSFERASE CATALYTIC DOMAIN.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-15 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 0.9796, 0.9800, 0.9324 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 95.670, 95.670, 112.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.500 - 2.800 |
| R-factor | 0.237 * |
| Rwork | 0.237 |
| R-free | 0.27700 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.075 | 0.120 |
| Number of reflections | 11867 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 95.7 | 95.7 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | cacodylate, magnesium chloride, sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | UMP | 10 (mM) | |
| 5 | 1 | drop | 500 (mM) | ||
| 6 | 1 | reservoir | sodium acetate | 1.3-1.6 (M) | |
| 7 | 1 | reservoir | cacodylate | 50 (mM) |
