1FG3
CRYSTAL STRUCTURE OF L-HISTIDINOL PHOSPHATE AMINOTRANSFERASE COMPLEXED WITH L-HISTIDINOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-31 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.484, 62.435, 45.530 |
| Unit cell angles | 90.00, 104.26, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.200 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.21800 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.072 | 0.213 |
| Total number of observations | 51924 * | |
| Number of reflections | 17053 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 92.6 | 68.7 |
| Redundancy | 3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 292 | PEG 3350, Tris-Hcl, MgCl2, Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 22.5 (%(w/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 7 | 1 | reservoir | 200 (mM) | ||
| 8 | 1 | reservoir | glycerol | 7 (%(w/v)) |
