1FG2

CRYSTAL STRUCTURE OF THE LCMV PEPTIDIC EPITOPE GP33 IN COMPLEX WITH THE MURINE CLASS I MHC MOLECULE H-2DB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-12-04
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	92.527, 124.770, 99.555
Unit cell angles	90.00, 103.03, 90.00

Refinement procedure

Resolution	30.000 - 2.754
Rwork	0.236
R-free	0.27600
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.900	2.810
High resolution limit [Å]	2.750	2.750
Rmerge	0.080	0.386
Number of reflections	51494	3331 *
<I/σ(I)>	11.7
Completeness [%]	90.8	88.4 *
Redundancy	2.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	4 *	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.85 (mg/ml)
2	1	reservoir	ammonium sulfate	0.1 (M)
3	1	reservoir	PEG4000	20 (%(w/v))
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir	sodium azide	0.02 (%(w/v))