1FFZ
LARGE RIBOSOMAL SUBUNIT COMPLEXED WITH R(CC)-DA-PUROMYCIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-02 |
| Detector | CUSTOM-MADE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 212.000, 300.000, 574.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.000 - 3.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.230 |
| High resolution limit [Å] | 3.000 | 3.170 |
| Rmerge | 0.188 | 0.840 |
| Total number of observations | 2590726 * | |
| Number of reflections | 367284 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.6 | 99.9 |
| Redundancy | 7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.1 * | 292 | Ban, N., (2000) Science, 289, 905. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | NH4Cl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | CdCl2 | ||
| 5 | 1 | 1 | potassium acetate | ||
| 6 | 1 | 1 | Tris-MES | ||
| 7 | 1 | 1 | PEG 6000 | ||
| 8 | 1 | 2 | PEG 6000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 3 | 1 | Kacetate | 100 (mM) | ||
| 5 | 1 | PEG6000 | 7 (%) | ||
| 6 | 1 | Tris | 15 (mM) | ||
| 7 | 1 | MES | 15 (mM) | ||
| 8 | 1 | 1 | 1 (mM) |
