1FFX

TUBULIN:STATHMIN-LIKE DOMAIN COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-22
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	328.500, 328.500, 54.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 3.950
R-factor	0.267
Rwork	0.267
R-free	0.36700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tub
RMSD bond length	0.012
RMSD bond angle	24.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.020
High resolution limit [Å]	3.950	3.950
Rmerge	0.062	0.333
Number of reflections	29784
<I/σ(I)>	18.9	2.9
Completeness [%]	99.1	98.7
Redundancy	4.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	PEG, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG