1FFS

CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM CRYSTALS SOAKED IN ACETYL PHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1997-10-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	159.300, 53.400, 77.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.217 *
Rwork	0.217
R-free	0.25500
RMSD bond length	0.014
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.064	0.200
Total number of observations	63874 *
Number of reflections	26184
<I/σ(I)>	11
Completeness [%]	96.5	98.1 *
Redundancy	2.4	2.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	297	Welch, M., (1998) Nature Struct. Biol., 5, 25. *