1FFP
CRYSTAL STRUCTURE OF MURINE CLASS I H-2DB COMPLEXED WITH PEPTIDE GP33 (C9M/K1S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-18 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.013, 66.897, 80.414 |
| Unit cell angles | 75.10, 72.90, 69.28 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| Rwork | 0.250 |
| R-free | 0.30300 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.094 | 0.357 * |
| Total number of observations | 109795 * | |
| Number of reflections | 25600 | |
| <I/σ(I)> | 9.01 | |
| Completeness [%] | 96.9 | 95.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 25% PEG 8000, 6% DMSO, 0.8M Glycine, 150mM NaCl 25mM MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | MES | 25 (mM) | pH6.5 |
| 3 | 1 | reservoir | PEG8000 | 10-20 (%) | |
| 4 | 1 | reservoir | MES | 25 (mM) | pH6.5 |
| 5 | 1 | reservoir | dioxane | 1 (%) |
