1FFP
CRYSTAL STRUCTURE OF MURINE CLASS I H-2DB COMPLEXED WITH PEPTIDE GP33 (C9M/K1S)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-18 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 |
Unit cell lengths | 47.013, 66.897, 80.414 |
Unit cell angles | 75.10, 72.90, 69.28 |
Refinement procedure
Resolution | 50.000 - 2.600 |
Rwork | 0.250 |
R-free | 0.30300 |
RMSD bond length | 0.008 |
RMSD bond angle | 25.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.094 | 0.357 * |
Total number of observations | 109795 * | |
Number of reflections | 25600 | |
<I/σ(I)> | 9.01 | |
Completeness [%] | 96.9 | 95.2 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 25% PEG 8000, 6% DMSO, 0.8M Glycine, 150mM NaCl 25mM MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MES | 25 (mM) | pH6.5 |
3 | 1 | reservoir | PEG8000 | 10-20 (%) | |
4 | 1 | reservoir | MES | 25 (mM) | pH6.5 |
5 | 1 | reservoir | dioxane | 1 (%) |