1FFH

N AND GTPASE DOMAINS OF THE SIGNAL SEQUENCE RECOGNITION PROTEIN FFH FROM THERMUS AQUATICUS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-03-19
Detector	MAR scanner 300 mm plate
Spacegroup name	C 1 2 1
Unit cell lengths	99.900, 53.910, 57.360
Unit cell angles	90.00, 119.77, 90.00

Refinement procedure

Resolution	20.000 - 2.050
R-factor	0.186
Rwork	0.186
R-free	0.24800
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	21.320 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.046 *	0.137 *
Number of reflections	16644
Completeness [%]	99.5	98.4
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9		PROTEIN AT 30 MG/ML IN WATER. CRYSTALLIZED AT RT BY SITTING DROP VAPOR DIFFUSION USING 30% PEG MME 550, 50 MM TAPS PH 9.0, 200 MM MGCL2., vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	mPEG550	30 (%)
3	1	reservoir	TAPS	100 (mM)
4	1	reservoir		200 (mM)