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1FFH

N AND GTPASE DOMAINS OF THE SIGNAL SEQUENCE RECOGNITION PROTEIN FFH FROM THERMUS AQUATICUS

Experimental procedure
Source typeROTATING ANODE
Source detailsRIGAKU
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1996-03-19
DetectorMAR scanner 300 mm plate
Spacegroup nameC 1 2 1
Unit cell lengths99.900, 53.910, 57.360
Unit cell angles90.00, 119.77, 90.00
Refinement procedure
Resolution20.000 - 2.050
R-factor0.186
Rwork0.186
R-free0.24800
Structure solution methodMIR
RMSD bond length0.006
RMSD bond angle21.320

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR (3.843)
Refinement softwareX-PLOR (3.843)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]2.120
High resolution limit [Å]2.0502.050
Rmerge0.046

*

0.137

*

Number of reflections16644
Completeness [%]99.598.4
Redundancy43.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP9PROTEIN AT 30 MG/ML IN WATER. CRYSTALLIZED AT RT BY SITTING DROP VAPOR DIFFUSION USING 30% PEG MME 550, 50 MM TAPS PH 9.0, 200 MM MGCL2., vapor diffusion - sitting drop
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein30 (mg/ml)
21reservoirmPEG55030 (%)
31reservoirTAPS100 (mM)
41reservoir200 (mM)

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