1FFG

CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY AT 2.1 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1997-10-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	157.600, 53.800, 76.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.188 *
Rwork	0.188
R-free	0.22000
RMSD bond length	0.014 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.046	0.151
Total number of observations	88506 *
Number of reflections	32997
<I/σ(I)>	15
Completeness [%]	85.2	79.3
Redundancy	2.7	2.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	297	Welch, M., (1998) Nature Struct. Biol., 5, 25. *