1FEU

CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-01
Detector	MARRESEARCH
Spacegroup name	P 31 1 2
Unit cell lengths	109.904, 109.904, 137.511
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.208 *
Rwork	0.208
R-free	0.24500
RMSD bond length	0.006
RMSD bond angle	1.070 *
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.052	0.365
Total number of observations	188440 *
Number of reflections	41886
<I/σ(I)>	17
Completeness [%]	98.8	97.8
Redundancy	5.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *		VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	7 *		VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2-4 (mg/ml)
2	1	drop	sodium cacodylate	10 (mM)
3	1	drop		100 (mM)
4	1	drop		4 (mM)
5	1	drop	MPD	7 (%)
6	1	reservoir		200 (mM)
7	1	reservoir	MPD	12 (%)