1FE8
CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-14 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.800, 183.600, 131.800 |
| Unit cell angles | 90.00, 116.20, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.030 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.061 | 0.404 |
| Number of reflections | 143255 * | |
| <I/σ(I)> | 20.4 | |
| Completeness [%] | 85.9 | 51.5 |
| Redundancy | 4.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 * | 4 * | iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 13 (%(v/v)) | precipitant |
| 3 | 1 | reservoir | MPD | 22 (%(v/v)) | precipitant |
| 4 | 1 | reservoir | cacodylic acid | 100 (mM) |
