1FE3

CRYSTAL STRUCTURE OF HUMAN BRAIN FATTY ACID BINDING PROTEIN OLEIC ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 65
Unit cell lengths	93.600, 93.600, 46.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.800
R-factor	0.19 *
Rwork	0.261
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015 *
RMSD bond angle	1.900 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.800
Rmerge	0.072 *
Total number of observations	11489 *
Number of reflections	5444 *
Completeness [%]	82.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *		VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8-2.2 (M)
2	1	reservoir	Tris	100 (mM)