1FDQ

CRYSTAL STRUCTURE OF HUMAN BRAIN FATTY ACID BINDING PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MACSCIENCE
Spacegroup name	P 1 21 1
Unit cell lengths	37.220, 87.930, 46.790
Unit cell angles	90.00, 113.40, 90.00

Refinement procedure

Resolution	15.000 - 2.100
Rwork	0.180
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015 *
RMSD bond angle	2.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.063 *
Total number of observations	102823 *
Number of reflections	21863
Completeness [%]	84.4
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.1 *	16 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	20 (%)
3	1	reservoir	Tris	0.1 (M)
4	1	reservoir		50 (mM)