1FDA

CRYSTAL STRUCTURES OF OXIDIZED AND REDUCED AZOTOBACTER VINELANDII FERREDOXIN AT PH 8 AND PH 6

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	55.200, 55.200, 95.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.206
Rwork	0.206
RMSD bond length	0.015
RMSD bond angle	3.210
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.062 *
Total number of observations	36781 *
Number of reflections	8487 *
Completeness [%]	93.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *	2 *	referred to J.Biol.Chem. 254.3598-3599 1979 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-10 (mg/ml)
2	1	drop	potassium phosphate	0.5 (M)
3	1	drop	ammonium sulfate	1.2 (M)
4	1	reservoir	ammonium sulfate	3.5 (M)