1FD9
CRYSTAL STRUCTURE OF THE MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN (MIP) A MAJOR VIRULENCE FACTOR FROM LEGIONELLA PNEUMOPHILA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.750, 80.750, 103.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.400 - 2.410 |
| R-factor | 0.2302 |
| Rwork | 0.230 |
| R-free | 0.28050 * |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.100 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.400 | 2.550 |
| High resolution limit [Å] | 2.410 | 2.600 * |
| Rmerge | 0.103 * | 0.428 * |
| Total number of observations | 52836 * | |
| Number of reflections | 10976 * | |
| <I/σ(I)> | 2.7 | 2.4 |
| Completeness [%] | 99.4 * | 99.7 * |
| Redundancy | 4.8 * | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 288 | PEG 8,000, zinc acetate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 100 (mM) | pH6.1-6.5 |
| 2 | 1 | reservoir | PEG8000 | 15-20 (%(w/v)) | |
| 3 | 1 | reservoir | zinc acetate | 500 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | HEPES | 20 (mM) | pH7.0 |
