1FD7
HEAT-LABILE ENTEROTOXIN B-PENTAMER WITH BOUND LIGAND BMSC001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-01 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.761, 157.538, 63.145 |
| Unit cell angles | 90.00, 116.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.187 |
| R-free | 0.23400 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.132 |
| Number of reflections | 79747 | |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 81.4 | 76 * |
| Redundancy | 2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 5000, NaCl, Tris/HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.0 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | BAPG | 2 (mM) | |
| 4 | 1 | reservoir | 50 (mM) | ||
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 6 | 1 | reservoir | PEG5000 | 30 (%(w/v)) |
