1FCP
FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI IN COMPLEX WITH BOUND FERRICHROME-IRON
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 171.400, 171.400, 85.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.300 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.079 * | 0.406 * |
| Number of reflections | 39633 | 4038 * |
| <I/σ(I)> | 16.5 | 2.3 |
| Completeness [%] | 98.9 | 98 |
| Redundancy | 5.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 | 100MM NA-CACODYLATE, PH 6.4, 12.5% PEG2000 MME, 20% GLYCEROL, 3% PEG200 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.4 |
| 3 | 1 | reservoir | PEG2000MME | 11 (%) | |
| 4 | 1 | reservoir | glycerol | 20 (%) | |
| 5 | 1 | reservoir | PEG200 | 3 (%) | |
| 6 | 1 | reservoir | dimethyldecylamine-N-oxide | 0.8 (%) | |
| 7 | 1 | reservoir | cis-inositol | 1 (%) |
