1FCK

STRUCTURE OF DICERIC HUMAN LACTOFERRIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	283
Detector technology	DIFFRACTOMETER
Collection date	1997-02-01
Detector	WEISSENBERG
Spacegroup name	P 21 21 21
Unit cell lengths	154.900, 97.100, 56.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.200
R-factor	0.202 *
Rwork	0.203
R-free	0.25690
RMSD bond length	0.006
RMSD bond angle	1.307
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.067	0.341
Number of reflections	35878 *
<I/σ(I)>	15.8
Completeness [%]	81.9	64.3
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	60 (mg/ml)
2	1	2	Tris-HCl	0.01 (M)
3	1	2	ethanol	10 (%(v/v))