1FCG
ECTODOMAIN OF HUMAN FC GAMMA RECEPTOR, FCGRIIA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE M18X |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-01 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.221, 100.866, 28.172 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.218 |
| Rwork | 0.197 |
| R-free | 0.24200 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.359 |
| Number of reflections | 15024 | |
| <I/σ(I)> | 10.3 | 1.5 |
| Completeness [%] | 94.9 | 95.9 |
| Redundancy | 2.73 | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 22 * | Powell, M.S., (1999) Immunol.Lett., 68, 17. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |
