1FCG

ECTODOMAIN OF HUMAN FC GAMMA RECEPTOR, FCGRIIA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	108
Detector technology	IMAGE PLATE
Collection date	1998-04-01
Detector	RIGAKU
Spacegroup name	P 21 21 2
Unit cell lengths	79.221, 100.866, 28.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.218
Rwork	0.197
R-free	0.24200
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.359
Number of reflections	15024
<I/σ(I)>	10.3	1.5
Completeness [%]	94.9	95.9
Redundancy	2.73	2.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	22 *	Powell, M.S., (1999) Immunol.Lett., 68, 17. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	ammonium sulfate	0.2 (M)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	PEG4000	30 (%(w/v))