1FCF
PHOTOSYSTEM II D1 C-TERMINAL PROCESSING PROTEASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 102 |
Detector technology | CCD |
Collection date | 1999-04-08 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 148.753, 148.753, 100.173 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.22 * |
Rwork | 0.220 |
R-free | 0.31800 |
RMSD bond length | 0.015 |
RMSD bond angle | 2.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.069 | 0.575 |
Total number of observations | 224983 * | |
Number of reflections | 25542 | |
<I/σ(I)> | 30.1 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | ammonium sulfate, lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3.5 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | |
3 | 1 | drop | phenylboronic acid | 1 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 30-40 (%(v/v)sat) | |
5 | 1 | reservoir | 0.2-0.4 (M) |