1FC9
PHOTOSYSTEM II D1 C-TERMINAL PROCESSING PROTEASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-03 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.600, 63.100, 60.700 |
| Unit cell angles | 90.00, 119.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.191 * |
| Rwork | 0.191 |
| R-free | 0.27500 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 0.482 |
| Total number of observations | 113233 * | |
| Number of reflections | 27619 | |
| <I/σ(I)> | 18.77 | |
| Completeness [%] | 97.8 | 95.1 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, isopropanol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | phenylboronic acid | 1 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 17-18 (%(w/v)) | |
| 5 | 1 | reservoir | isopropanol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
