1FC7
PHOTOSYSTEM II D1 C-TERMINAL PROCESSING PROTEASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.800, 64.100, 63.400 |
| Unit cell angles | 90.00, 122.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.187 * |
| Rwork | 0.187 |
| R-free | 0.26700 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.548 |
| Total number of observations | 112278 * | |
| Number of reflections | 25755 | |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.3 | 85.7 |
| Redundancy | 4.36 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, isopropanol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | phenylboronic acid | 1 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 17-18 (%(w/v)) | |
| 5 | 1 | reservoir | isopropanol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
