1FC6
PHOTOSYSTEM II D1 C-TERMINAL PROCESSING PROTEASE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 102 |
| Detector technology | CCD |
| Collection date | 1998-11-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.300, 64.450, 63.730 |
| Unit cell angles | 90.00, 122.11, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.168 * |
| Rwork | 0.168 |
| R-free | 0.23700 |
| Structure solution method | MAD, treating MAD data as a special case of MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.394 |
| Total number of observations | 258532 * | |
| Number of reflections | 35489 | |
| <I/σ(I)> | 18 | |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 7.28 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 4000, isopropanol, HEPES, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | phenylboronic acid | 1 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 17-18 (%(w/v)) | |
| 5 | 1 | reservoir | isopropanol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
