1FC3
THE CRYSTAL STRUCTURE OF TRANS-ACTIVATION DOMAIN OF THE SPORULATION RESPONSE REGULATOR, SPO0A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-12-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.118, 53.414, 53.718 |
| Unit cell angles | 90.82, 111.73, 111.32 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.1991 |
| Rwork | 0.196 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 * | 0.313 * |
| Number of reflections | 27976 | |
| <I/σ(I)> | 19.41 | 3.23 |
| Completeness [%] | 87.6 | 55.6 |
| Redundancy | 3.6 * | 1.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 289 * | Muchova, K., (1999) Acta Crystallogr., D55, 671. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | 50 (mM) | ||
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) |
