1FC0
HUMAN LIVER GLYCOGEN PHOSPHORYLASE COMPLEXED WITH N-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-16 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 31 |
| Unit cell lengths | 124.000, 124.000, 122.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fa9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.047 | 0.174 |
| Number of reflections | 81720 | |
| <I/σ(I)> | 22.5 | |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 5.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 290 | NaMES, MPD, N-acetyl-beta-D-glycopyranosylamine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 17K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-40 (mg/ml) | |
| 2 | 1 | drop | NaBES | 20 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | N-acetyl-beta-D-glucopyranosylamine | 30-80 (mM) | |
| 6 | 1 | reservoir | sodium MES | 0.1 (M) | |
| 7 | 1 | reservoir | MPD | 25-35 (%) |
