1FBY
CRYSTAL STRUCTURE OF THE HUMAN RXR ALPHA LIGAND BINDING DOMAIN BOUND TO 9-CIS RETINOIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 1999-04-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.325, 86.358, 90.527 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.250 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.350 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.045 | 0.230 |
| Total number of observations | 87788 * | |
| Number of reflections | 22453 | |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 98.9 | 95.5 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 22 * | used micro and macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 2-4.5 (M) | |
| 2 | 1 | reservoir | Tris | 50 (mM) | |
| 3 | 1 | reservoir | glycerol | 5-10 (%) | |
| 4 | 1 | reservoir | propane-1,2-diol | 17-23 (%) |
