1FBU
HEAT SHOCK TRANSCRIPTION FACTOR DNA BINDING DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.800, 64.100, 50.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.750 - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26700 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.830 |
| Rmerge | 0.080 |
| Total number of observations | 53481 * |
| Number of reflections | 13067 |
| <I/σ(I)> | 16.5 |
| Completeness [%] | 85.4 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 100 mM sodium acetate pH 4.6, 200 mM ammonium acetate, 24-28% polyethylene glycol average molecular weight 4000, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 22-30 (%) | |
| 2 | 1 | reservoir | ammonium acetate | 50-200 (mM) | |
| 3 | 1 | reservoir | citrate | 100 (mM) | pH5.6-6.2 |
