1FBP

CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATASE COMPLEXED WITH FRUCTOSE 6-PHOSPHATE, AMP, AND MAGNESIUM

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	61.600, 166.600, 80.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.500
R-factor	0.215 *
Rwork	0.215
RMSD bond length	0.013
RMSD bond angle	3.500
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.061 *
Total number of observations	87690 *
Number of reflections	27511 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	AMP	1 (mM)
2	1	1	F6P	1 (mM)
3	1	1		5 (mM)
4	1	1	Fru-1,6-Pase
5	1	2	Tris	20 (mM)
6	1	2	maleic acid	2 (mM)
7	1	2	EDTA	0.1 (mM)
8	1	2		5 (mM)
9	1	2	PEG3350	9.5 (%(w/v))