1FBN

CRYSTAL STRUCTURE OF A FIBRILLARIN HOMOLOGUE FROM METHANOCOCCUS JANNASCHII, A HYPERTHERMOPHILE, AT 1.6 A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	170
Collection date	1997-10-01
Wavelength(s)	0.97921,0.97894,0.96373
Spacegroup name	C 1 2 1
Unit cell lengths	121.407, 43.264, 55.303
Unit cell angles	90.00, 96.99, 90.00

Refinement procedure

Resolution	12.000 - 1.600
R-factor	0.229 *
Rwork	0.229
R-free	0.24800
Structure solution method	MIRAS
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.076 *
Number of reflections	37155
Completeness [%]	97.0	95.8
Redundancy	2	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6		20% 2-PROPANOL, 20% PEG4k, 0.1M SODIUM CITRATE, pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	PEG4000	10 (%)
3	1	drop	isopropanol	10 (%)
4	1	drop	sodium citrate	0.05 (M)
5	1	reservoir	PEG4000	20 (%)
6	1	reservoir	isopropanol	20 (%)
7	1	reservoir	sodium citrate	0.1 (M)