1FBM

ASSEMBLY DOMAIN OF CARTILAGE OLIGOMERIC MATRIX PROTEIN IN COMPLEX WITH ALL-TRANS RETINOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-12-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.470, 49.470, 54.980
Unit cell angles	90.00, 103.84, 90.00

Refinement procedure

Resolution	3.000 - 2.700
Rwork	0.195
RMSD bond length	0.001
RMSD bond angle	18.680 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	3.000	3.000
High resolution limit [Å]	2.700	2.700
Rmerge	0.132
Total number of observations	20313 *
Number of reflections	5464
Completeness [%]	86.0	87.6
Redundancy	3.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	COMPcc (16 mg/ml) in 20 mM sodium phosphate, 200 mM NaCl (pH 7.5) precipitant: 7-8% PEG 8000, 50 mM sodium cacodylate (pH 6.0), 50 mM sodium acetate, 50 mM calcium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 20.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	reservoir	PEG8000	7-8 (%)
3	1	reservoir	sodium cacodylate	50 (mM)
4	1	reservoir	sodium acetate	50 (mM)
5	1	reservoir	calcium acetate	50 (mM)