1FBK
CRYSTAL STRUCTURE OF CYTOPLASMICALLY OPEN CONFORMATION OF BACTERIORHODOPSIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ELECTRON MICROSCOPE |
| Source details | OTHER |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1998-12-01 |
| Detector | OTHER |
| Spacegroup name | P 3 |
| Unit cell lengths | 62.450, 62.450, 100.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 200.000 - 3.200 |
| Rwork | 0.272 |
| R-free | 0.32100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 200.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.173 |
| Number of reflections | 5668 |
| Completeness [%] | 77.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 * | 4 * | crystals are increased in size by fusion and annealing using detergents, pH 7, naturally occurring in vivo, temperature 37K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 18-23 (mg/ml) | |
| 2 | 1 | 1 | beta-octylglucopyranoside | 0.5 (%(w/v)) | |
| 3 | 1 | 1 | benzamidine | 4 (%(w/v)) | |
| 4 | 1 | 1 | sodium phosphate | 1.75 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.8-2.3 (M) |
