1FBH

CRYSTALLOGRAPHIC STUDIES OF THE CATALYTIC MECHANISM OF THE NEUTRAL FORM OF FRUCTOSE-1,6-BISPHOSPHATASE

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	131.200, 131.200, 69.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 2.500
R-factor	0.193
Rwork	0.193
RMSD bond length	0.012
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.077 *
Total number of observations	94675 *
Number of reflections	24277 *
Completeness [%]	91.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10-15 (mg/ml)
2	1	2	Tris base	20 (mM)
3	1	2	maleic acid	2 (mM)
4	1	2	EDTA	0.1 (mM)
5	1	2		5 (mM)
6	1	2	2-mercapthoethanol	1 (mM)
7	1	2	PEG3350	7.5 (%(w/v))
8	1	2	F6P	1 (mM)