1FBE

CRYSTALLOGRAPHIC STUDIES OF THE CATALYTIC MECHANISM OF THE NEUTRAL FORM OF FRUCTOSE-1,6-BISPHOSPHATASE

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	132.000, 132.000, 67.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 3.000
R-factor	0.195
Rwork	0.195
RMSD bond length	0.018
RMSD bond angle	3.820
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Rmerge	0.063 *
Total number of observations	54498 *
Number of reflections	1332 *
Completeness [%]	90.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10-15 (mg/ml)
2	1	2	Tris base	20 (mM)
3	1	2	maleic acid	2 (mM)
4	1	2	EDTA	0.1 (mM)
5	1	2		5 (mM)
6	1	2	2-mercapthoethanol	1 (mM)
7	1	2	PEG3350	7.5 (%(w/v))
8	1	2	F6P	1 (mM)