1FB8
STRUCTURE OF THE PLECKSTRIN HOMOLOGY DOMAIN FROM DAPP1/PHISH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-10 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | I 4 |
| Unit cell lengths | 83.380, 83.380, 38.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.400 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.27200 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.140 |
| Total number of observations | 17635 * | |
| Number of reflections | 4973 | |
| <I/σ(I)> | 21.5 | 5.2 |
| Completeness [%] | 94.0 | 81.3 * |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | PEG 3450, Ethylene Glycol, Ammonium Phosphate, Sodium Phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3450 | 15-20 (%) | |
| 2 | 1 | reservoir | ethylene glycol | 20-30 (%) | |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | ammonium phosphate | 200 (mM) | |
| 5 | 1 | drop | sodium phosphate | 25 (mM) | |
| 6 | 1 | drop | protein | 25 (mg/ml) |
