1FB5

LOW RESOLUTION STRUCTURE OF OVINE ORNITHINE TRANSCARBMOYLASE IN THE UNLIGANDED STATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-10
Detector	MARRESEARCH
Spacegroup name	P 43 3 2
Unit cell lengths	184.700, 184.700, 184.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.500
R-factor	0.219
Rwork	0.212
R-free	0.25000 *
RMSD bond length	0.010
RMSD bond angle	23.400 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	3.920
High resolution limit [Å]	3.500	3.510
Rmerge	0.160	0.320
Number of reflections	13959
<I/σ(I)>	4.3
Completeness [%]	98.2	99.5
Redundancy	10	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	298	8% PEG 4000, 0.1 M Na acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-9 (mg/ml)
2	1	reservoir	Tris-HCl	10 (mM)	pH7.4
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	PEG4000	8 (%(w/v))	pH4.6