1FAS

1.9 ANGSTROM RESOLUTION STRUCTURE OF FASCICULIN 1, AN ANTI-ACETYLCHOLINESTERASE TOXIN FROM GREEN MAMBA SNAKE VENOM

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	40.300, 40.300, 81.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.149
Rwork	0.149
RMSD bond length	0.015
RMSD bond angle	2.770
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.062 *
Total number of observations	27118 *
Number of reflections	4874 *
Completeness [%]	84.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		100 (mM)
3	1	reservoir		20 (%sat)
4	1	reservoir	MES	100 (mM)