1FAA

CRYSTAL STRUCTURE OF THIOREDOXIN F FROM SPINACH CHLOROPLAST (LONG FORM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	293
Detector technology	DIFFRACTOMETER
Collection date	1991
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 1 21 1
Unit cell lengths	30.600, 63.100, 31.600
Unit cell angles	90.00, 110.70, 90.00

Refinement procedure

Resolution	25.600 - 1.850
R-factor	0.209 *
Rwork	0.209
R-free	0.26800
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	ROTAVATA
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.600
High resolution limit [Å]	1.850
Rmerge	0.035
Number of reflections	9180
Completeness [%]	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Genovesio-Taverne, J.C., (1991) J. Mol. Biol., 222, 459. *