1FA0
STRUCTURE OF YEAST POLY(A) POLYMERASE BOUND TO MANGANATE AND 3'-DATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-04 |
Detector | OTHER |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.800, 109.100, 238.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.27800 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.044 | 0.307 |
Total number of observations | 242159 * | |
Number of reflections | 58367 | |
<I/σ(I)> | 32.36 | |
Completeness [%] | 97.8 | 99.9 |
Redundancy | 4.14 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.24 | 4 * | PEG,Ethylene Glycol, pH 7.24, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ethylene glycol | 13.5 (%) | |
2 | 1 | reservoir | PEG8000 | 9 (%) | |
3 | 1 | reservoir | glycerol | 6 (%) | |
4 | 1 | reservoir | HEPES | 100 (mM) | |
5 | 1 | reservoir | dithiothreitol | 2 (mM) | |
6 | 1 | drop | protein | 35 (mg/ml) |