1FA0
STRUCTURE OF YEAST POLY(A) POLYMERASE BOUND TO MANGANATE AND 3'-DATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-04 |
| Detector | OTHER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.800, 109.100, 238.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.27800 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.044 | 0.307 |
| Total number of observations | 242159 * | |
| Number of reflections | 58367 | |
| <I/σ(I)> | 32.36 | |
| Completeness [%] | 97.8 | 99.9 |
| Redundancy | 4.14 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.24 | 4 * | PEG,Ethylene Glycol, pH 7.24, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ethylene glycol | 13.5 (%) | |
| 2 | 1 | reservoir | PEG8000 | 9 (%) | |
| 3 | 1 | reservoir | glycerol | 6 (%) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 6 | 1 | drop | protein | 35 (mg/ml) |
