1F9T

CRYSTAL STRUCTURES OF KINESIN MUTANTS REVEAL A SIGNALLING PATHWAY FOR ACTIVATION OF THE MOTOR ATPASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-01-01
Spacegroup name	P 1 21 1
Unit cell lengths	43.610, 78.810, 47.170
Unit cell angles	90.00, 105.01, 90.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.213 *
Rwork	0.213
R-free	0.24400
RMSD bond length	0.006
RMSD bond angle	1.199
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.074
Total number of observations	793427 *
Number of reflections	47889
<I/σ(I)>	16.2
Completeness [%]	97.3	94.9
Redundancy	16.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	18 *	HEPES, PEG 2000 ME, magnesium chloride, Ethylen glycol, Sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG2000 ME	20-26 (%)
3	1	reservoir		0.2 (M)
4	1	reservoir	HEPES	50 (mM)