1F9S
CRYSTAL STRUCTURE OF PLATELET FACTOR 4 MUTANT 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-17 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.400, 77.480, 42.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.380 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.28800 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.092 | 0.899 |
| Number of reflections | 10165 | |
| <I/σ(I)> | 25.1 | |
| Completeness [%] | 90.9 | 47.2 |
| Redundancy | 10.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | PEG 1000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
