1F9M

CRYSTAL STRUCTURE OF THIOREDOXIN F FROM SPINACH CHLOROPLAST (SHORT FORM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-10-02
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	46.590, 52.560, 52.570
Unit cell angles	90.00, 108.93, 90.00

Refinement procedure

Resolution	25.500 - 1.860
R-factor	0.197 *
Rwork	0.198
R-free	0.23700
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.500	1.960
High resolution limit [Å]	1.860	1.860
Rmerge	0.082	0.237
Number of reflections	20015
<I/σ(I)>	5.7
Completeness [%]	98.9	95.3
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3 *	20 *	ammonium sulphate, sodium acetate, PEG 4000, pH 5.80, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		0.2 (M)
4	1	drop		0.02 (%(w/v))
5	1	reservoir	ammonium sulfate	0.2 (M)
6	1	reservoir	sodium acetate	0.1 (M)
7	1	reservoir	PEG4000	25 (%(w/v))