1F9G
CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE COCRYSTALLIZED WITH ASCORBIC ACID
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-24 |
Detector | BRANDEIS - B4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.264, 102.666, 103.253 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.000 |
Rwork | 0.216 |
R-free | 0.25200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.087 | 0.320 |
Number of reflections | 54324 | |
<I/σ(I)> | 11.7 | |
Completeness [%] | 90.1 | 60 * |
Redundancy | 14.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6 | 293 | ammonium sulfate, sodium cacodylate, dioxane, sodium chloride, ascorbic acid , pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | ascorbic acid | 10-100 (mM) |